Antibacterial Resistance Pattern of Pseudomonas aeruginosa Isolated from Clinical Samples at a General Hospital in Padang, West Sumatra, Indonesia

Abstract

Objectives: Pseudomonas aeruginosa is an opportunistic pathogen that has an innate resistance to some antibiotics. This bacterium is one of the most common causes of nosocomial infections that include surgical wound infections, burns, and urinary tract infections. The bacteria have been reportedly resistant some antibiotics, and have developed multi-drug resistance. The objective of the study was to determine the pattern resistance of Pseudomonas aeruginosa isolated from clinical samples of patients against some major antibiotics. Methods: Isolates of P. aeruginosa were obtained from the clinical sample of urine, sputum, swabs, pus, feces, and blood and cultured in cetrimide agar (CA). P. aeruginosa ATCC 27853 was used as a positive control. The antibacterial susceptibility testing was conducted against 13 antibiotics: ceftazidime, cefotaxime, ceftriaxone, cefoperazone, ciprofloxacin, levofloxacin, ofloxacin, gentamicin, amikacin, piperacillin, ticarcillin, meropenem, and imipenem. The examination was carried out by using agar diffusion method of Kirby-Bauer and following CLSI Standards. Results: The results showed that bacterial resistance was established against all tested antibiotics. The highest number of resistance was shown against ceftriaxone (44.21%), while the most susceptibility was exhibited against amikacin (only 9.47% of resistance). Multidrug-resistance Pseudomonas aeruginosa (MDRPA) was detected in almost all clinical samples tested, except the feces. The sample with the highest percentage of MDRPA was the pus. Conclusion: She study revealed that the most effective antibiotic against Pseudomonas aeruginosa was amikacin (91.51%), while the most resistance is exhibited against ceftriaxone (43.16%).

Full paper

Cite this article: Rustini R, Jamsari J, Marlina M, Zubir N, Yuliandra Y. Antibacterial Resistance Pattern of Pseudomonas aeruginosa Isolated from Clinical Samples at a General Hospital in Padang, West Sumatra, Indonesia. Asian J Pharm Clin Res 2017; 10(8): 158-160.

DOI: 10.22159/ajpcr.2017.v10i8.18539