Reversible Hepatotoxicity of Cassytha filiformis Extract: Experimental Study on Liver Function and Propofol-Induced Sleep in Mice
- At January 20, 2019
- By Yori Yuliandra
- In Scientific Paper
45
Yori Yuliandra, Armenia Armenia*, Rahmad Arief, Mifta Hul Jannah, Helmi Arifin
Abstract
Objective: To study the liver toxicity of Cassytha filiformis L. extract and its reversibility in mice. Methods: A total of 108 male mice were used to investigate the hepatotoxicity of Cassytha filiformis extract (CFE) and the reversibility of its toxicity to the liver. Seventy-two mice were treated with CFE orally for 7 consecutive days. A half of these animals were used to study the sleep time on propofol-induced sleep that comprised the sleep onset time (SOT) and duration of sleep (DOS), while another half was used for the quantitation of serum alanine transaminase (ALT) activity. To investigate the reversibility of the liver toxicity, thirty-six mice were treated with daily CFE for 7 days. Activities of ALT and alkaline phosphatase (ALP) were determined and the liver weight ratio was measured on day 0, 1, 3 and 7 after the termination of the CFE treatment. Data of liver toxicity determination were analyzed by two-way ANOVA followed by Duncan’s multiple range test, while data of the reversibility was analyzed by Pearson’s correlation. The significance level was taken at 95% of confidence interval. Results: CFE shortened the SOT and prolonged the DOS significantly compared with control (p<0.05). The activity of ALT was increased due to the toxicity of CFE. However, the ALT/ALP activities decreased and liver weight ratio increased gradually after the extract treatment was discontinued. The trend of these data was correlated significantly (p<0.05). Conclusion: Cassytha filiformis L. extract is toxic to the liver but the toxicity is reversible depending on doses.
Read More»Antihypertensive and antioxidant activity of Cassytha filiformis L.: A correlative study
- At August 02, 2017
- By Yori Yuliandra
- In Scientific Paper
- 45
Objective: To study the blood pressure lowering effect of Cassytha filiformis extract in animal models of hypertension and its correlation with the antioxidant activity. Method: Male Sprague-Dawley rats were divided into two groups: endocrine hypertension (HTN group) that received a combination of prednisone and salt for two weeks and oxidative stress-associated hypertension (HTN-OS group) that received additional induction of L-Nitro Arginine Methyl Esther (L-NAME) for two days. Each group was subdivided into 4 and treated intravenously with the extract 5; 10; and 20 mg/kg, and vehicle control. The systolic blood pressure (SBP), diastolic blood pressure (DBP), and mean arterial pressure (MAP) were recorded. The blood was taken before and at the end of recording for the measurement of serum concentration of nitric oxide (NO). The changes of blood pressure were analyzed by two-way ANOVA while its correlation with NO concentration was analyzed by Pearson’s Correlation. Results: The study showed a significant antihypertensive effect of the extract as compared with control group (P<0.05) in both hypertensive models. Extract in the dose of 5 mg/kg showed the best blood pressure lowering effect. However, the correlation analysis did not show an association between NO increase and blood pressure lowering effect (P>0.05). Conclusion: The study concludes that Cassytha filiformis extract in the dose of 5 mg/kg exhibits the best blood pressure lowering effect in both animal models. Antihypertensive activity of the extract is not correlated with its antioxidant effect.
Read More»Uji Toksisitas Subkronis Ekstrak Etanol Tali Putri (Cassytha filiformis L.) Terhadap Fungsi Ginjal Tikus
- At December 30, 2015
- By Yori Yuliandra
- In Scientific Paper
- 0
Sub-chronic toxicity test of the deffated ethanolic extract of Cassytha filiformis L. on the renal function of rat has been carried out. A number of 16 male rats aged 2-3 months, weighing ±250 g were used as experimental animals. The rats were divided into 4 groups which consisted of 1 control group and 3 extract-treated groups given 1.25, 2.5, and 5 mg/kg extract administrated intraperitoneally once a day for 14 days. The creatinine clearance, renal function, and the ratio of renal were calculated and analyzed by two-way ANOVA. The study showed that the creatinine clearance and renal function decreased significantly during treatment (p<0.05). The kidney weight ratio of groups treated with extract, especially with greater doses, showed a very significant increase as compared with control (p<0,01). These indicated that the ethanolic extract of C. filiformis on the doses of 1.25 – 5 mg/kg might decrease the renal function, althought it seemed to be relatively safe when used for 14 days.
Read More»Acute and Delayed Toxicity Study of Cassytha Filiformis Defatted Ethanolic Extract
- At October 14, 2015
- By Yori Yuliandra
- In Scientific Paper
- 0
Defatted ethanolic extract of Cassytha filiformis exhibits a potential antihypertensive activity based on our previous study. The objective of this study is to evaluate the acute and delayed toxicities of the extract. Determination of LD 50 and delayed toxicity were conducted on 90 mice (divided into 6 groups of 15 mice each) at doses of 25, 50, 100, 200, 400 and 800 mg/kg. Data was analyzed by Probit analysis to determine LD 50 , while Two Way ANOVA was used to analyze the change of animal body weight, food and water intakes. Results showed that the 1, 2, 3, 24 and 48 hours LD 50 of this extract were 0; 875.83; 313. 63; 52.28 and 2.17 mg/kg, respectively. Animal body weight, food and water intakes were affected by the dose of the extract and the time of measurement and interaction of those variables significantly (p<0.05). In this situation, food and water intake decreased up to fifteen days after the single dose of this extract, especially at high dose (400 mg). These led to reducing animal body weight. There was no significant change (p>0.1) in the heart, liver and kidney weight ratios at the end of this study (14 days after single dose of extract). These indicate that the defatted ethanolic extract of C. filiformis is toxic and produces delayed toxicity.
Read More»Efek Antihipertensi Tumbuhan Tali Putri (Cassytha filiformis L.) pada Tikus Hipertensi Yang Diinduksi Prednison dan Garam
- At June 28, 2013
- By Yori Yuliandra
- In Scientific Paper
- 0
A study of the antihypertensive effect of Cassytha filiformis L. defatted extract on the hypertensive rats has been carried out. The study was conducted on 30 male Sprague-Dawley rats induced by Prednisone-NaCl for 14 days to obtain hypertensive rats. All rats were anesthetized to perform direct blood pressure measurement and divided into control group, extract-treated groups (5, 10 and 20 mg/kg), and tempol group. Each rat received three repetitive doses administered intravenously in one hour interval. During each dose interval, the systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), and heart rate (HR) were measured. All cardiovascular data were presented as mean of percentage change ± SEM. The data were analyzed by three-way ANOVA and Tukey’s HSD. The significance level was taken at P<0.05. The study showed that the defatted extract of C. filiformis decreased SBP, DBP, MAP, and HR significantly. The extract at the dose of 5 mg/kg and tempol 100 mcmol/kg showed insignificant difference in lowering blood pressure. Keywords: cassytha filiformis, tali putri, antihypertensive, blood pressure, heart rate
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